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Afterwards, Cys 63 nucleophilically attacks this sulfide releasing a GS - anion, thereby creating the second GSH

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Methylcobalamin, on the other hand, is a naturally occurring form of B12

Performance of the method The modified GPx-DTNB method's performance was completed following the bioanalytical method validation guidelines set by the Committee for Medicinal Products for Human Use (Singh and Narsinghani 2023)