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After 30 min blocking with 1x PTW/0.5% BSA (Bovine Serum Albumin) solution, add primary antibody and incubate for 2 hours at room temperature or overnight at 4C

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Patients with autoimmune hypothyroidism who have appropriate indications such as type 2 diabetes or obesity may safely use these medications under medical supervision, provided they do not have a personal or family history of medullary thyroid carcinoma or MEN 2 syndrome

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The liver is where excess B12 is stored and is used to regulate B12 levels

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