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Protocol 4: nasal spray approach Goal: Needle-free administration with acceptable bioavailability trade-off Duration: 20 days active, then 4-6 months off Schedule: Days 1-20: 10-15 mg epitalon intranasally, divided into 2-3 daily doses Days 21-180: Off cycle Repeat 1-2 times per year Formulation: Reconstitute in saline with 3 percent DMSO for improved nasal absorption

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The cells in control well were not treated with FOXO4-DRI, and the blank well containing the medium without cells were used to normalized

392 :e086886 doi:10.1136/bmj-2025-086886 Vlker KM, Prechtl BLH, Bormann NL, Choi DS

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