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Expression and purification of Fab7F38GLP-1RsemaglutideGs Human GLP-1R (Arg24-Leu422) fragment fused with a N-terminal HA-FLAG-B2-HRV14-3C tags and C-terminal HPC4-HRV14-3C-10 His tags was synthesized and inserted into a modified pFastBac-1 vector (BamHI/HindIII sites) under the polh promoter (Genewiz) (see primers in Supplementary Table 2)

These data suggest hypermetabolism and metabolic inefficiency in aUC colonoids at the early stages of differentiation which is associated with cellular stress

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Piezo1 channels restrain ILC2s and regulate the development of airway hyperreactivity

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